APPLICATION ID 361

Mephobarbital

COMPOUND IDENTIFICATION

Compound Name Mephobarbital
Mephobarbital

CHROMATOGRAM

Chromatogram

ANALYTICAL REPORT

Column Trade Name

Column Size / Particle Size

CHIRALPAK® IA

250 x 4.6mm / 5µm

Mobile Phase Composition

n-hexane / ethyl acetate = 30 / 70

Chromatographic Mode

Normal Phase
Secondary screening solvent (Immobilised CSP)

Flow rate (ml/min)

1

Injection amount (mg)

0.01

Temperature (°C)

25

Pressure (MPa)

Detection

ELSD

Elution order

No.

Rt

K’

1

3.87

0.29

2

7.08

1.36

Alpha Value

Resolution

1 - 2

4.69

16.6

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