APPLICATION ID 363

Mephobarbital

COMPOUND IDENTIFICATION

Compound Name Mephobarbital
Mephobarbital

CHROMATOGRAM

Chromatogram

ANALYTICAL REPORT

Column Trade Name

Column Size / Particle Size

CHIRALPAK® IB

250 x 4.6mm / 5µm

Mobile Phase Composition

n-hexane / 2-propanol = 90 / 10

Chromatographic Mode

Normal Phase
Primary screening solvent (Immobilised CSP)

Flow rate (ml/min)

1

Injection amount (mg)

0.01

Temperature (°C)

25

Pressure (MPa)

Detection

UV 230 nm

Elution order

No.

Rt

K’

1

8.64

1.89

2

12.1

3.06

Alpha Value

Resolution

1 - 2

1.61

9.49

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