APPLICATION ID 378

Metoprolol

COMPOUND IDENTIFICATION

Compound Name Metoprolol
Metoprolol

CHROMATOGRAM

Chromatogram

ANALYTICAL REPORT

Column Trade Name

Column Size / Particle Size

CHIRALPAK® IB

250 x 4.6mm / 5µm

Mobile Phase Composition

n-hexane / chloroform / ethanolamine = 65 / 35 / 0.1

Chromatographic Mode

Normal Phase
Secondary screening solvent (Immobilised CSP)

Flow rate (ml/min)

1

Injection amount (mg)

0.008

Temperature (°C)

25

Pressure (MPa)

Detection

UV 270 nm

Elution order

No.

Rt

K’

1

6.84

2.28

2

9.64

3.22

Alpha Value

Resolution

1 - 2

1.41

8.25

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