APPLICATION ID 383

N-3,5-DNB-DL-Leucine

COMPOUND IDENTIFICATION

Compound Name N-3,5-DNB-DL-Leucine
N-3,5-DNB-DL-Leucine

CHROMATOGRAM

Chromatogram

ANALYTICAL REPORT

Column Trade Name

Column Size / Particle Size

CHIRALPAK® IB

250 x 4.6mm / 5µm

Mobile Phase Composition

n-hexane / 2-propanol / trifluoroacetic acid = 75 / 25 / 0.1

Chromatographic Mode

Normal Phase
Primary screening solvent (Immobilised CSP)

Flow rate (ml/min)

1

Injection amount (mg)

0.01

Temperature (°C)

25

Pressure (MPa)

Detection

UV 254 nm

Elution order

No.

Rt

K’

1

9.83

2.34

2

14.63

3.98

Alpha Value

Resolution

1 - 2

1.7

5.96

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