APPLICATION ID 40

DL-4-Nitrophenylalanine

COMPOUND IDENTIFICATION

Compound Name DL-4-Nitrophenylalanine
DL-4-Nitrophenylalanine

CHROMATOGRAM

Chromatogram

ANALYTICAL REPORT

Column Trade Name

Column Size / Particle Size

CHIRALPAK® ZWIX(+)

150 x 3mm / 3µm

Mobile Phase Composition

25mM formic acid + 25mM diethylamine in methanol / acetonitrile / water = 49 / 49 / 2

Chromatographic Mode

Polar Phase

Flow rate (ml/min)

0.5

Injection amount (mg)

0.005

Temperature (°C)

25

Pressure (MPa)

6.6

Detection

ELSD

Elution order

No.

Rt

K’

1

7.32

3.19

2

8.58

3.91

Alpha Value

Resolution

1 - 2

1.23

3.09

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