ID
452

Tramadol

COMPOUND IDENTIFICATION

Molecular Weight
263.374
Formula
C16H25O2N
Property
Basic
Tramadol

Compound name

Tramadol

CHROMATOGRAM

Tramadol

ANALYTICAL REPORT

Column Trade Name
(Column size / Particle size)
CHIRALPAK® IC
(4.6 x 250 mm 5 µm)
Chromatographic Mode Normal Phase
Mobile Phase Composition n-hexane / tetrahydrofuran / diethylamine = 85 / 15 / 0.1
Flow rate (mL/min) 1 Temperature (℃) 25
Injection Volume (µL) 0.01 (mg) Sample Concentration
Detection UV-VIS 270 nm
Elution Order
No. Rt K'
1 4.1 0.35
2 4.8 0.56
Alpha Value Resolution
1 - 2 1.59 5.14