APPLICATION ID 452

Tramadol

COMPOUND IDENTIFICATION

Compound Name Tramadol
Tramadol

CHROMATOGRAM

Chromatogram

ANALYTICAL REPORT

Column Trade Name

Column Size / Particle Size

CHIRALPAK® IC

250 x 4.6mm / 5µm

Mobile Phase Composition

n-hexane / tetrahydrofuran / diethylamine = 85 / 15 / 0.1

Chromatographic Mode

Normal Phase
Primary screening solvent (Immobilised CSP)

Flow rate (ml/min)

1

Injection amount (mg)

0.01

Temperature (°C)

25

Pressure (MPa)

Detection

UV 270 nm

Elution order

No.

Rt

K’

1

4.13

0.35

2

4.76

0.56

Alpha Value

Resolution

1 - 2

1.59

5.14

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