APPLICATION ID 472

Atropine

COMPOUND IDENTIFICATION

Compound Name Atropine
Atropine

CHROMATOGRAM

Chromatogram

ANALYTICAL REPORT

Column Trade Name

Column Size / Particle Size

CHIRALPAK® AY-3

150 x 4.6mm / 3µm

Mobile Phase Composition

n-hexane / ethanol / diethylamine = 80 / 20 / 0.1

Chromatographic Mode

Normal Phase
Coated CSP

Flow rate (ml/min)

1

Injection amount (mg)

0.005

Temperature (°C)

25

Pressure (MPa)

Detection

UV 230 nm

Elution order

No.

Rt

K’

1

4.16

1.35

2

5.17

1.92

Alpha Value

Resolution

1 - 2

1.42

5.39

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