APPLICATION ID 473

Bisoprolol

COMPOUND IDENTIFICATION

Compound Name Bisoprolol
Bisoprolol

CHROMATOGRAM

Chromatogram

ANALYTICAL REPORT

Column Trade Name

Column Size / Particle Size

CHIRALPAK® AY-3

150 x 4.6mm / 3µm

Mobile Phase Composition

n-hexane / 2-propanol / diethylamine = 80 / 20 / 0.1

Chromatographic Mode

Normal Phase
Coated CSP

Flow rate (ml/min)

1

Injection amount (mg)

0.005

Temperature (°C)

25

Pressure (MPa)

Detection

UV 230 nm

Elution order

No.

Rt

K’

1

4.35

1.46

2

5.76

2.26

Alpha Value

Resolution

1 - 2

1.55

5.85

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