APPLICATION ID 505

Secobarbital

COMPOUND IDENTIFICATION

Compound Name Secobarbital
Secobarbital

CHROMATOGRAM

Chromatogram

ANALYTICAL REPORT

Column Trade Name

Column Size / Particle Size

CHIRALPAK® AY-3

150 x 4.6mm / 3µm

Mobile Phase Composition

n-hexane / ethanol = 95 / 5

Chromatographic Mode

Normal Phase
Coated CSP

Flow rate (ml/min)

1

Injection amount (mg)

0.005

Temperature (°C)

25

Pressure (MPa)

Detection

UV 210 nm

Elution order

No.

Rt

K’

1

6.66

2.76

2

7.42

3.19

Alpha Value

Resolution

1 - 2

1.16

2.35

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