APPLICATION ID 525

Atropine

COMPOUND IDENTIFICATION

Compound Name Atropine
Atropine

CHROMATOGRAM

Chromatogram

ANALYTICAL REPORT

Column Trade Name

Column Size / Particle Size

CHIRALCEL® OZ-H

250 x 4.6mm / 5µm

Mobile Phase Composition

n-hexane / 2-propanol / diethylamine = 80 / 20 / 0.1

Chromatographic Mode

Normal Phase
Coated CSP

Flow rate (ml/min)

1

Injection amount (mg)

0.01

Temperature (°C)

25

Pressure (MPa)

Detection

UV 230 nm

Elution order

No.

Rt

K’

1

7.89

1.62

2

11.84

2.94

Alpha Value

Resolution

1 - 2

1.81

7.42

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