APPLICATION ID 679

Ofloxacin

COMPOUND IDENTIFICATION

Compound Name Ofloxacin
Ofloxacin

CHROMATOGRAM

Chromatogram

ANALYTICAL REPORT

Column Trade Name

Column Size / Particle Size

CHIRALPAK® AS-H

250 x 4.6mm / 5µm

Mobile Phase Composition

n-hexane / ethanol / acetic acid / ethylenediamine = 50 / 50 / 0.3 / 0.3

Chromatographic Mode

Normal Phase
Coated CSP

Flow rate (ml/min)

1

Injection amount (mg)

Temperature (°C)

40

Pressure (MPa)

Detection

UV 300 nm

Elution order

No.

Rt

K’

1

5.73

0.91

2

7.52

1.51

Alpha Value

Resolution

1 - 2

1.66

4.54

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