APPLICATION ID 708

Warfarin

COMPOUND IDENTIFICATION

Compound Name Warfarin
Warfarin

CHROMATOGRAM

Chromatogram

ANALYTICAL REPORT

Column Trade Name

Column Size / Particle Size

CHIRALPAK® AD-H

250 x 4.6mm / 5µm

Mobile Phase Composition

n-heptane / ethanol / acetic acid = 50 / 50 / 0.1

Chromatographic Mode

Normal Phase
Coated CSP

Flow rate (ml/min)

1

Injection amount (mg)

0.01

Temperature (°C)

25

Pressure (MPa)

Detection

UV 230 nm

Elution order

No.

Rt

K’

1

3.94

0.31

2

5.84

0.95

Alpha Value

Resolution

1 - 2

3.03

7.1

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