APPLICATION ID 735

N-3,5-DNB-DL-Leucine

COMPOUND IDENTIFICATION

Compound Name N-3,5-DNB-DL-Leucine
N-3,5-DNB-DL-Leucine

CHROMATOGRAM

Chromatogram

ANALYTICAL REPORT

Column Trade Name

Column Size / Particle Size

CHIRALPAK® IB-3

50 x 4.6mm / 3µm

Mobile Phase Composition

trifluoroacetic acid aq. pH2.0 / acetonitrile = 50 / 50

Chromatographic Mode

Reverse Phase

Flow rate (ml/min)

5

Injection amount (mg)

0.005

Temperature (°C)

25

Pressure (MPa)

Detection

UV 254 nm

Elution order

No.

Rt

K’

1

0.35

1.89

2

0.6

3.97

Alpha Value

Resolution

1 - 2

2.1

5.68

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