APPLICATION ID 746

Warfarin

COMPOUND IDENTIFICATION

Compound Name Warfarin
Warfarin

CHROMATOGRAM

Chromatogram

ANALYTICAL REPORT

Column Trade Name

Column Size / Particle Size

CHIRALPAK® IB-3

150 x 4.6mm / 3µm

Mobile Phase Composition

trifluoroacetic acid aq. pH2.0 / acetonitrile = 60 / 40

Chromatographic Mode

Reverse Phase

Flow rate (ml/min)

1

Injection amount (mg)

0.005

Temperature (°C)

25

Pressure (MPa)

Detection

UV 270 nm

Elution order

No.

Rt

K’

1

11.19

5.39

2

13.86

6.92

Alpha Value

Resolution

1 - 2

1.28

7.23

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