APPLICATION ID 800

Warfarin

COMPOUND IDENTIFICATION

Compound Name Warfarin
Warfarin

CHROMATOGRAM

Chromatogram

ANALYTICAL REPORT

Column Trade Name

Column Size / Particle Size

CHIRALCEL® OD-3R

50 x 4.6mm / 3µm

Mobile Phase Composition

formic acid aq. pH2.0 / acetonitrile = 40 / 60

Chromatographic Mode

Reverse Phase

Flow rate (ml/min)

3

Injection amount (mg)

0.005

Temperature (°C)

25

Pressure (MPa)

Detection

UV 270 nm

Elution order

No.

Rt

K’

1

0.43

1.29

2

0.56

2.02

Alpha Value

Resolution

1 - 2

1.56

1.85

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