APPLICATION ID 917

N-3,5-DNB-DL-Leucine

COMPOUND IDENTIFICATION

Compound Name N-3,5-DNB-DL-Leucine
N-3,5-DNB-DL-Leucine

CHROMATOGRAM

Chromatogram

ANALYTICAL REPORT

Column Trade Name

Column Size / Particle Size

CHIRALPAK® HSA

100 x 4mm / 5µm

Mobile Phase Composition

10mM NH4OAc aq. (+5mM heptafluorobutyric acid) pH7.0 / 2-propanol = 85 / 15

Chromatographic Mode

Reverse Phase

Flow rate (ml/min)

0.9

Injection amount (mg)

0.0005

Temperature (°C)

20

Pressure (MPa)

Detection

UV 225 nm

Elution order

No.

Rt

K’

1

6.96

5.46

2

10.28

8.54

Alpha Value

Resolution

1 - 2

1.56

2.08

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