COMPOUND IDENTIFICATION
Compound name
- N-3,5-DNB-DL-Leucine
CHROMATOGRAM
ANALYTICAL REPORT
|
Column Trade Name (Column size / Particle size) |
CHIRALPAK® HSA (4 x 100 mm 5 µm) |
|---|---|
| Chromatographic Mode | Reverse Phase |
| Mobile Phase Composition | 10mM NH4OAc aq. (+5mM heptafluorobutyric acid) pH7.0 / 2-propanol = 85 / 15 |
| Flow rate (mL/min) | 0.9 | Temperature (℃) | 20 |
|---|---|---|---|
| Injection Volume (µL) | 0.0005 (mg) | Sample Concentration | |
| Detection | UV-VIS 225 nm | ||
| Elution Order | |||
| No. | Rt | K' |
|---|---|---|
| 1 | 7.0 | 5.46 |
| 2 | 10.3 | 8.54 |
| Alpha Value | Resolution | |
|---|---|---|
| 1 - 2 | 1.56 | 2.08 |